N,NKa taea te whakamahi i te -bis(2-chloroethyl)carbamate tert-butyl ester hei takawaenga i roto i te matū rongoā me te hanga rauropi. I roto i ngā panonitanga hanga rauropi, ka taea te tango ngāwari i te rōpū Boc i roto i te hanganga hei whiwhi i tētahi rōpū carboxyl hohe.

Ko te tikanga hanga ko te whakaoho tere i te whakatārewatanga o te bis-(2-chloroethyl)amine hydrochloride (5 g, 0.028 mol) i roto i te dichloromethane (42 mL) me te10% te wairewa konutai hauwai (28 mL) i te pāmahana kaukau hukapapa. He wairewa o te di-tert-butyl dicarbonate (6.1Kātahi ka tāpirihia he 1 karamu, 0.028 mol) ki roto i te dichloromethane (28 mChemicalbook L). Whai muri i te whakaoho i te pāmahana rūma mō ngā hāora 18.5, ka tāpirihia mārire te dichloromethane (30 mL) ki te ranunga tauhohenga. Kātahi ka wehea ngā āhua e rua. Ka tangohia anō te āhua wai mā te dichloromethane (30 mL). Ka whakamaroketia ngā paparanga rauropi kua whakakotahitia ki runga i te konupora whanariki kore wai, ka tātarihia, ka whakapūmauhia kia whiwhi ai i te N,N-bis(2-chloroethyl)carbamate tert-butyl.